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KMID : 0380620190510040316
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Korean Journal of Food Science and Technology
2019 Volume.51 No. 4 p.316 ~ p.323
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ement of analytical method for pymetrozine in citrus fruits
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Jeon Jun-Ho
Chun Su-Hyun
Kim Min-Hyuk
Kim Mi-Ok
Lee Kwang-Won
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Abstract
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It is difficult to analyze pymetrozine in citrus fruits using the hydromatrix method because of its low efficiency of purification and overlap of matrix and pymetrozine peaks. Liquid-liquid extraction can analyze pymetrozine in citrus fruits using dichloromethane. Since low pH interferes with the extraction of pymetrozine, the extracts of citrus fruits were maintained over pH 7.0 by adding borax buffer and 1 N NaOH in the improved method. According to the improved method, citrus fruits (such as lemon, lime, orange, tangerine, and grapefruit) were extracted and purified for HPLC-photo diode array analysis. The results of validation were as follows: 4.360 μg/kg of limit of detection, 14.533 μg/kg of limit of quantitation, and 0.007 mg/kg of method quantitative limit. Citrus fruits spiked with pymetrozine showed a recovery range from 71.8 to 83.7% and a coefficient of variation below 6%. Thus, the improved method can efficiently analyze pymetrozine in citrus fruits.
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KEYWORD
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pymetrozine, analytical method, HPLC-PDA, citrus fruits, liquid-liquid extraction
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